Polymerase chain reaction (PCR) technique compared to conventional bacteriological and serological techniques in diagnosis of human brucellosis in Egypt
نویسندگان
چکیده
منابع مشابه
Diagnosis of human brucellosis in Egypt by polymerase chain reaction.
This study aimed to establish an accurate and sensitive polymerase chain reaction (PCR) technique for the diagnosis of active human brucellosis in Egypt. We failed to extract Brucella DNA with a commercial kit, but an extraction kit designed in-house using 2 sets of primers [B4/B5 (223 bp) and JPF/JPR (193 bp)] was successful and more economical. The technique showed high sensitivity, specifici...
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The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR ...
متن کاملComparison of Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Agglutination Assays in Diagnosis of Brucellosis in Golestan Province, North of Iran
Introduction: Brucellosis is one of the most common zoonotic infections worldwide. The clinical symptoms of brucellosis are similar to a wide range of diseases; hence, reliable diagnostic and laboratory methods are required to identify the causative agent. Iran is an endemic region of brucellosis, and many patients are misdiagnosed due to the nature of the infection. In this study, we aimed to ...
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چکیده ندارد.
15 صفحه اولComparison of nasopharyngeal culture, polymerase chain reaction (PCR) and serological test for diagnosis of pertussis.
This prospective study, which was designed to compare nasopharyngeal culture, polymerase chain reaction (PCR) and serology in the diagnosis of pertussis, covered 35 children aged between 0 and 16 who were admitted to Hacettepe University Ihsan Doğramaci Children's Hospital between 1 March 2005 and 31 August 2006 with coughing for 7 days or longer, paroxysmal cough of any duration, or cough with...
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ژورنال
عنوان ژورنال: African Journal of Microbiology Research
سال: 2016
ISSN: 1996-0808
DOI: 10.5897/ajmr2015.7851